Product Description

Phospho-TNK2-Y284 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79412-50, BS79412-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07912

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HepG2 cells, using Phospho-TNK2-Y284 antibody at 1:1000 dilution. HepG2 cells were treated by EGF for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 180kDa

Specificity: Phosphorylated

Alternative Name: TNK2; ACK; ACK-1; ACK1; p21cdc42Hs

Immunogen: A synthetic phosphorylated peptide around Y284 of human TNK2(NP_005772.3).

Conjugate: Unconjugated

Modification: Phosphorylated