Product Description

PI 3-kinase p85α Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3199-50, ES3199-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha. AA range:573-622

Isotype: IgG

Target: PI3 Kinase P85α

Specificity: PI 3-kinase p85α Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein.

Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle, Isoform 2 and isoform 4 are present in skeletal muscle (at protein level),

Observed Band (KD): 85kD

Fields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, Platinum drug resistance, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, cAMP signaling pathway, Chemokine signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Phosphatidylinositol signaling system, Sphingolipid signaling pathway, Phospholipase D signaling pathway, Autophagy - animal, mTOR signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Apoptosis, Longevity regulating pathway, Longevity regulating pathway - multiple species, Cellular senescence, Axon guidance, VEGF signaling pathway, Osteoclast differentiation, Focal adhesion, Signaling pathways regulating pluripotency of stem cells, Platelet activation, Neutrophil extracellular trap formation, Toll-like receptor signaling pathway, C-type lectin receptor signaling pathway, JAK-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, >

Function: Disease: Defects in PIK3R1 are a cause of severe insulin resistance
Domain: The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef
Function: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues
PTM: Polyubiquitinated in T-cells by CBLB, which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation
Similarity: Belongs to the PI3K p85 subunit family
Similarity: Contains 1 Rho-GAP domain
Similarity: Contains 1 SH3 domain
Similarity: Contains 2 SH2 domains
Subunit: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat

Subcellular Location: nucleus, cytoplasm, cis-Golgi network, cytosol, plasma membrane, cell-cell junction, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA, membrane, perinuclear endoplasmic reticulum membrane,

Storage and Stability: -20°C/1 year

Gene Name: PIK3R1

Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha

Mouse Gene ID: 18708

Mouse SwissProt Number: P26450

Rat Gene ID: 25513

Rat SwissProt Number: Q63787

Research Use Only