Product Description

PI3K p85 beta Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66566-50, MB66566-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3, 4, 5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4, 5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regμlatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regμlatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regμlatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins. Protein extracts from 3T3-Src cells were profiled by PhosphoScan® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB

Alternate Names: Phosphatidylinositol 3-kinase regμlatory subunit beta, PI3-kinase regμlatory subunit beta, PI3K regμlatory subunit beta, PtdIns-3-kinase regμlatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regμlatory subunit beta, PI3-kinase subunit p85-beta, PtdIns-3-kinase regμlatory subunit p85-beta

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human PI3 Kinase p85 beta protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of PI3K p85 beta protein.

Molecular Weight: ~ 85 kDa

Pathway: AMPK Signaling Pathway，Regμlation of Actin Dynamics，Tumor Angiogenesis，Regμlation of Apoptosis，ESC Pluripotency and Differentiation Signaling Pathway，ErbB HER Signaling，Jak Stat: IL-6 Receptor Signaling，Inhibition of Apoptosis，Regμlation of Microtubμle Dynamics，Mitochondrial Control of Apoptosis，NF-kB Signaling，PI3K AKT signaling，Protein Kinase C Signaling，T Cell Receptor Signaling，Warburg Effect，mTOR Signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00459

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only