Pig AP (Aprotinin) ELISA Kit - ELK5919

Pig AP (Aprotinin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5919-48, ELK5919-96, ELK5919-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

Assay Type: Competitive Inhibition

Sensitivity: 0.46 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Cardiovascular biology; Human gene deletion; Hepatology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only