Product Description

Pig COL3 (Collagen Type III) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5714-48, ELK5714-96, ELK5714-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Pig

Alternate Names: Collagen Type 3, Col 3, Collagen Type III, Col III

Sensitivity: 0.64 ng/mL

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolic Pathway, Cardiovascular Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only