PIK3R3/p55PIK Polyclonal Antibody-BS77220

PIK3R3/p55PIK Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77220-50, BS77220-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92569

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:100

Background: Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 60kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PIK3R3/p55PIK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of NIH-3T3 cells using PIK3R3/p55PIK antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using PIK3R3/p55PIK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PIK3R3; p55; p55-GAMMA; p55PIK

Immunogen: Recombinant fusion protein of human PIK3R3/p55PIK(NP_003620.3).

Conjugate: Unconjugated

Modification: Unmodified