Product Description

PINCH (3C12) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3154-50, MB3154-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P48059

Host: Mouse

Reactivity: Human

Applications: WB, ICC/IF, FC, IP,

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 37 kDa; Observed MW: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunoprecipitation analysis of PINCH in Hela lysates using PINCH antibody., Western blot analysis of PINCH in C6, Jurkat, K562, 3T3 and Hela lysates using PINCH antibody, Immunofluorescence analysis of PINCH in HepG2 cells using PINCH antibody and DAPI., Flow cytometry analysis of Hela stained with PINCH antibody and negative control.

Alternative Name: LIMS1; PINCH; PINCH1; LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48

Immunogen: Purified recombinant fragment of human PINCH expressed in E. Coli.

Conjugate: Unconjugated

Modification: Unmodified