Product Description

PINK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79658-50, BS79658-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BXM7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 50KDa/63KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Rat liver, using PINK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Western blot analysis of extracts of various cell lines, using PINK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunofluorescence analysis of A549 cells using PINK1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining.

Alternative Name: PINK1; BRPK; PARK6

Immunogen: Recombinant fusion protein of human PINK1(NP_115785.1).

Conjugate: Unconjugated

Modification: Unmodified