Product Description

PKC α (phospho-T638) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4346-50, BS4346-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Members of the protein kinase C (PKC) family play a key regμlatory role in a variety of cellμlar functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 638.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-PKC á/â2 (T638) polyclonal antibody detects endogenous levels of PKC á/â2 protein when phosphorylated at Thr638.

Molecular Weight: ~ 80 kDa

Pathway: Phospholipase Signaling, Protein Kinase C Signaling, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17252

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only