Plant Root Activity Assay Kit
Size: 100 Assays
Catalogue Number: E-BC-K855-S-100
Citations, Manuals and MSDS Available upon request.
Research Areas: Plant Physiology Series
Result Type: Enzyme Activity
Detection Instrument: Spectrophotometer
Tested Sample Types: plant tissue
Assay Time: 3.5h
Detection Type: Colorimetric method
Sensitivity: 0 U/g wet weight
Detection Range: 0-0.67 U/g wet weight
Test Principle: The detection principle of the kit is to use 2，3，5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for 含量 determination of plant root activity or dehydrogenase activity.
Inter CV: 3
Intra CV: 1
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag