Product Description

PLC γ2 (phospho Tyr753) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1477-50, ES1477-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PLCG2, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG2 around the phosphorylation site of Tyr753. AA range:721-770

Isotype: IgG

Target: PLCG2

Specificity: Phospho-PLC γ2 (Y753) Polyclonal Antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Y753.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Lymph, Lymphoblast, Spleen, T-cell,

Observed Band (KD): 148kD

Fields: Inositol phosphate metabolism, Metabolic pathways, EGFR tyrosine kinase inhibitor resistance, ErbB signaling pathway, Ras signaling pathway, Calcium signaling pathway, Chemokine signaling pathway, NF-kappa B signaling pathway, HIF-1 signaling pathway, Phosphatidylinositol signaling system, Phospholipase D signaling pathway, Axon guidance, VEGF signaling pathway, Osteoclast differentiation, Platelet activation, Neutrophil extracellular trap formation, C-type lectin receptor signaling pathway, Natural killer cell mediated cytotoxicity, B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Inflammatory mediator regulation of TRP channels, Thyroid hormone signaling pathway, AGE-RAGE signaling pathway in diabetic complications, Growth hormone synthesis, secretion and action, Vibrio cholerae infection, Epithelial cell signaling in Helicobacter py

Function: Catalytic Activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Cofactor: Calcium
Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling
PTM: Phosphorylated on tyrosine residues, upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity
Similarity: Contains 1 C2 domain
Similarity: Contains 1 PH domain
Similarity: Contains 1 PI-PLC X-box domain
Similarity: Contains 1 PI-PLC Y-box domain
Similarity: Contains 1 SH3 domain
Similarity: Contains 2 SH2 domains

Subcellular Location: intracellular, cytosol, plasma membrane, extracellular exosome,

Storage and Stability: -20°C/1 year

Gene Name: PLCG2

Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Mouse Gene ID: 234779

Mouse SwissProt Number: Q8CIH5

Rat Gene ID: 29337

Rat SwissProt Number: P24135

Research Use Only