Product Description

PP1α (G315) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1765-50, BS1765-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62136

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are 37 kDa MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the 16-18 kDa inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of PP1α (G315) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 37 kDa

Specificity: PP1α (G315) polyclonal antibody detects endogenous levels of PP1α protein.

Alternative Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A; PPP1CA; PPP1A

Immunogen: Synthetic peptide, corresponding to amino acids 280-330 of Human PP1α.

Conjugate: Unconjugated

Modification: Unmodified