Product Description

PP1α (G315) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1765-50, BS1765-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regμlating a broad range of cellμlar functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regμlatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are 37 kDa MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the 16-18 kDa inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II resμlts in the release of active PP1.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 280-330 of Human PP1α.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PP1α (G315) polyclonal antibody detects endogenous levels of PP1α protein.

Molecular Weight: ~ 37 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P62136

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only