Product Description

PRAS40 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67171-50, MB67171-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which resμlts in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimμlates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insμlin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB

Alternate Names: PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human PRAS40. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/2000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of PRAS40 protein.

Molecular Weight: ~ 40 kDa

Pathway: Autophagy Signaling, PI3K AKT signaling, T Cell Receptor Signaling, Translational Contral elF4 and p70 S6 Kinase, mTOR Signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96B36

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only