Product Description

Prolactin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66016-50, MB66016-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P01236

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: The anterior pituitary secretes a variety of hormones that are involved in cell growth, differentiation and development. Prolactin, a 226 amino acid protein, plays a role in multiple processes, including cell growth, reproduction and immune function. Full length Prolactin, as well as an alternative splice product lacking the third exon, are secreted by endothelial cells involved in angiogenesis. In addition to its role in mammary developmant and lactation, Prolactin is known to play a role in the development of mammary cancer, acting as both a mitogen and a differentiating agent. Prolactin has also been shown to enhance the proliferation of B cell hybridomas, leading to an overall increase in antibody production. In addition, Prolactin has been demonstrated to reverse the antiproliferative effects of the immunosuppressive cytokine TGF-b. Prolactin is also associated with a variety of autoimmune diseases, including arthritis and type 1 diabetes.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of Prolactin staining in human hyperprolaceinemia formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 25 kDa

Specificity: Recognizes endogenous levels of Prolactin protein.

Alternative Name: Prolactin; PRL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Prolactin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified