Product Description

PSMB10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7781-50, BS7781-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40306

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:100

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 29kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of HeLa cells using PSMB10 antibody. Blue: DAPI for nuclear staining.

Alternative Name: PSMB10; LMP10; MECL1; beta2i

Immunogen: Recombinant fusion protein of human PSMB10(NP_002792.1).

Conjugate: Unconjugated

Modification: Unmodified