Product Description

PUS10 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES13856-50, ES13856-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA, IHC

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human PUS10 AA range: 425-475

Isotype: IgG

Target: PUS10

Specificity: This antibody detects endogenous levels of PUS10 at Human/Mouse

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Molecular Weight (Da): 58kD

Function: Catalytic Activity: tRNA uridine = tRNA pseudouridine
Function: Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Sequence Caution: Partially unspliced pre-RNA
Similarity: Belongs to the pseudouridine synthase Pus10 family

Subcellular Location: Nucleus Cytoplasm Mitochondrion Localizes mainly in the nucleus (Probable) (PubMed:31819270) tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270) Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101)

Storage and Stability: -20°C/1 year

Gene Name: PUS10 CCDC139 DOBI

Protein Name: PUS10

Mouse Gene ID: 74467

Mouse SwissProt Number: Q9D3U0

Research Use Only