Product Description

Pyruvate carboxylase (PC) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78747-50, BS78747-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11498

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100 | IP,1:50 - 1:200

Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 130KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using Pyruvate carboxylase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Immunofluorescence analysis of U2OS cells using Pyruvate carboxylase) antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Pyruvate carboxylase) antibody. Western blot was performed from the immunoprecipitate using Pyruvate carboxylase) antibody at a dilution of 1:1000.

Alternative Name: PC; PCB

Immunogen: Recombinant fusion protein of human Pyruvate carboxylase (Pyruvate carboxylase (PC))(NP_001035806.1).

Conjugate: Unconjugated

Modification: Unmodified