Pyruvate carboxylase (PC) Rabbit pAb - A6301

Pyruvate carboxylase (PC) Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A6301-20, A6301-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

Category: Polyclonal Antibodies

Applications: WB, IF/ICC, IP, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 130kDa

Observed Molecular Weight: 130kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human

GeneID: 5091

SWISS: P11498

Alternate Names: PCB; Pyruvate carboxylase (PC)

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:1000 IF/ICC, 1:10 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Lipid Metabolism, Carbohydrate metabolism.

NCBI Alias: PC

Research Use Only