Product Description

RAB8A Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES10133-50, ES10133-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malign

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 120-200

Isotype: IgG

Target: RAB8A

Specificity: RAB8A Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Brain, Epithelium, Platelet, Skin,

Observed Band (KD): 22kD

Fields: Autophagy - animal, Endocytosis, AMPK signaling pathway, Tight junction, Pancreatic secretion, Amyotrophic lateral sclerosis, Pathways of neurodegeneration - multiple diseases

Function: Function: May be involved in vesicular trafficking and neurotransmitter release
Similarity: Belongs to the small GTPase superfamily. Rab family
Subunit: Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3

Subcellular Location: Cell membrane, Lipid-anchor, Cytoplasmic side Golgi apparatus Recycling endosome membrane Cell projection, cilium Cytoplasmic vesicle, phagosome Cytoplasmic vesicle, phagosome membrane, Lipid-anchor, Cytoplasmic side Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasm, cytoskeleton, cilium basal body Midbody Cytoplasm Cytoplasm, cytoskeleton, cilium axoneme Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803) In the GDP-bound form, present in the perinuclear region (PubMed:12221131) Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131) Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical me

Storage and Stability: -20°C/1 year

Gene Name: RAB8A MEL RAB8

Protein Name: Ras-related protein Rab-8A (Oncogene c-mel)

Mouse SwissProt Number: P55258

Rat SwissProt Number: P35280

Research Use Only