Rabbit S100A12 (S100 Calcium Binding Protein A12) ELISA Kit - ELK8293

Rabbit S100A12 (S100 Calcium Binding Protein A12) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8293-48, ELK8293-96, ELK8293-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

Assay Type: Sandwich

Sensitivity: 0.066 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Infection immunity; Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit S100A12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit S100A12 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O77791

Research Use Only