Product Description

Rabbit S100A12 (S100 Calcium Binding Protein A12) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8293-48, ELK8293-96, ELK8293-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit S100A12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit S100A12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CGRP, P6, CAAF1, CAGC, ENRAGE, MRP6, Calgranulin C, Calcium-binding protein in amniotic fluid 1, Extracellular newly identified RAGE-binding, Migration inhibitory factor-related 6

Reactivity: Rabbit

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Infection Immunity, Immune Molecule

Assay Length: 3.5h

Sensitivity: 0.066 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: O77791

Research Use Only