Product Description

Rac 1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67052-50, MB67052-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regμlation, cell growth and development . GTP binding stimμlates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regμlation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state .

Category: Primary Antibody

Reactivity: Human, Rat

Host: Mouse

Applications: WB, IHC, FC

Alternate Names: TC25, Ras-related C3 botμlinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide of human Rac 1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), FC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Rac 1 protein.

Molecular Weight: ~ 24 kDa

Pathway: Regμlation of Actin Dynamics, Adherens Junction signaling, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway, Regμlation of Microtubμle Dynamics, SAPK JNK Signaling Cascades, T cell receptor signaling, TGFβ Smad signaling, Wntβ catenin signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P63000

Product: Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only