Product Description

RAD51A Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66512-50, MB66512-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human disease, including cancer. DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 . BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR. Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk. Some studies have implicated Rad51 as a potential marker for pancreatic cancer.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Applications: WB

Alternate Names: RAD51A, RECA, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein of human Rad51.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of RAD51A protein.

Molecular Weight: ~ 37 kDa

Pathway: Immune checkpoint signaling in the Tumor Microenvironment

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q06609

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only