Product Description

RALB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8542-50, BS8542-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11234

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200

Background: Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RALB polyclonal antibody detects endogenous levels of RALB protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RALB antibody at 1:1000 dilution., Immunohistochemistry of paraffin-embedded human liver damage using RALB antibody at dilution of 1:100 (40x lens)., Immunohistochemistry of paraffin-embedded human colon carcinoma using RALB antibody at dilution of 1:100 (40x lens)., Immunohistochemistry of paraffin-embedded human gastric cancer using RALB antibody at dilution of 1:100 (40x lens).

Alternative Name: Ras-related protein Ral-B; RALB

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RALB (NP_002872.1).

Conjugate: Unconjugated

Modification: Unmodified