RAPH1 Polyclonal Antibody-BS76344

RAPH1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76344-50, BS76344-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q70E73

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:200 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 200kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RAPH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of C6 cells using RAPH1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using RAPH1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: RAPH1; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RMO1; RalGDS/AF-6

Immunogen: Recombinant fusion protein of human RAPH1(NP_998754.1).

Conjugate: Unconjugated

Modification: Unmodified