Rat GCK (Glucokinase) ELISA Kit - ELK2776

Rat GCK (Glucokinase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2776-48, ELK2776-96, ELK2776-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase 4; Maturity Onset Diabetes Of The Young 2

Assay Type: Sandwich

Sensitivity: 0.24 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway; Endocrinology; Nutrition metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GCK in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P17712

Research Use Only