Rat HMGA1 (High Mobility Group AT Hook Protein 1) ELISA Kit - ELK9443

Rat HMGA1 (High Mobility Group AT Hook Protein 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9443-48, ELK9443-96, ELK9443-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HMG-R; HMGIY; High mobility group protein A1; High mobility group protein R; High mobility group protein HMG-I/HMG-Y

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Tumor immunity; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8K585

Research Use Only