Product Description

Rat IL-12p40 (Interleukin 12 p40) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK12013-48, ELK12013-96, ELK12013-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: IL-12B, p40, IL12B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit

Sensitivity: 3.2 pg/mL

Assay Type: Sandwich

Standard: 300 pg/mL

Detection Range: 4.69-300 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cytokine, Infection Immunity, Immune Molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only