Rat IL7R (Interleukin 7 Receptor) ELISA Kit - ELK5749

Rat IL7R (Interleukin 7 Receptor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5749-48, ELK5749-96, ELK5749-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CDW127; CD127; IL-7R-Alpha

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; CD and Adhesion molecule; Infection immunity; Immunodeficiency;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL7R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL7R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL7R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL7R in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only