Product Description

Rat IRS1 (Insulin Receptor Substrate 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8434-48, ELK8434-96, ELK8434-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: HIRS1

Sensitivity: 0.131 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IRS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IRS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IRS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IRS1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P35570

Research Use Only