Product Description

Rb Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16670-50, BZ16670-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 106 kDa; Observed MW: 106 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Retinoblastoma in Jurkat lysates; MCF-7 lysates using Rb antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Rb in SH-SY5Y using Retinoblastoma antibody.

Alternative Name: RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Immunogen: A synthesized peptide derived from human Retinoblastoma

Conjugate: Unconjugated

Modification: Unmodified