Product Description

RBCK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78025-50, BS78025-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BYM8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 58kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RBCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of U2OS cells using RBCK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using RBCK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using RBCK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3

Immunogen: Recombinant fusion protein of human RBCK1(NP_112506.2).

Conjugate: Unconjugated

Modification: Unmodified