Product Description

RBFOX1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78926-50, BS78926-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NWB1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:100 - 1:200

Background: The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 45-50KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using RBFOX1 antibody at 1:849 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of mouse brain cells using RBFOX1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of rat brain cells using RBFOX1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: RBFOX1; 2BP1; A2BP1; FOX-1; FOX1; HRNBP1

Immunogen: Recombinant fusion protein of human RBFOX1(NP_665900.1).

Conjugate: Unconjugated

Modification: Unmodified