Recombinant Human AIM2 Protein (GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031042-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: AIM2
Target Symptom: PYHIN4
Research Area: Cancer; epigenetics and nuclear signal
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Cell culture
Fusion Tag: N-GST
Accession: NP_004824.1
Background: AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-200 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.
Activity: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-40ug/ml.
Sequence: Met 1-Thr 343
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 1M NaCl, 0.5mM PMSF, 5mM GSH, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 65.2 kDa
Observed MW: 65.2 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only