Recombinant Human APE1/APE Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032090-10, PKSH032090-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: APE1; APE
Target Synonym: DNA-(Apurinic or Apyrimidinic Site) Lyase; APEX Nuclease; APEN; Apurinic-Apyrimidinic Endonuclease 1; AP Endonuclease 1; APE-1REF-1; Redox Factor-1; APEX1; APE; APE1; APEX; APX; HAP1; REF1
UNIProt ID: P27695
Research Areas: Cardiovascular; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
Accession: P27695
Background: Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases; APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors; such as c-Jun; c-Fos; NF-kB; p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.
Sequence: Pro2-Leu318
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 35.6 kDa
Observed MW: 40 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only