Recombinant Human CA5A/CA-VA Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031568-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CA5A; CA-VA
Target Symptom: CA5; CA5AD; CA5D; Carbonic Anhydrase VA; CAV; CAVA; GS1-21A4.1
Research Area: Signal Transduction; Tags & Cell Markers; metabolism;
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: C-His
Accession: NP_001730.1
Background: Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
Activity: Measured by its esterase activity. The specific activity is > 500 pmoles/min/μg.
Sequence: Ala 40-Ser 305
Purity: > 96 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 31.6 kDa
Observed MW: 33 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only