Recombinant Human CD155/PVR Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031868-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD155; PVR
Target Symptom: CD155; HVED; Necl-5; NECL5; PVS; TAGE4
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_006496.3
Background: CD155; commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5); is a type I transmembrane single-span glycoprotein; and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV); an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension; migration and NK-cell killing; and thus efficiently prime cell-mediated tumor-specific immunity.
Activity: Immobilized human CD155 at 2 μg/ml (100 μl/well) can bind human DNAM1 with a linear ranger of 1. 28-32 ng/ml.
Sequence: Met 1-Asn 343
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 36.5 kDa
Observed MW: 60-65 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only