Recombinant Human CDKN1B Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032314-10, PKSH032314-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CDKN1B
Target Synonym: Cyclin-Dependent Kinase Inhibitor 1B; Cyclin-Dependent Kinase Inhibitor p27; p27Kip1; CDKN1B; KIP1
UNIProt ID: P46527
Research Areas: Cell biology; Cancer; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P46527
Background: Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) is a Kinesin-related motor protein necessary for mitotic spindle assembly and chromosome segregation. CDKN1B is expressed in all tissues with highest levels observed in skeletal muscle. CDKN1B is a potent inhibitor of Cyclin E- and Cyclin A-CDK2 complexes. CDKN1B forms a complex with Cyclin Type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. In addition, CDKN1B acts as an inhibitor or an activator of Cyclin Type D-CDK4 complexes depending on its phosphorylation state and stoichometry.
Sequence: Met 1-Thr198
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 24.2 kDa
Observed MW: 27 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only