Recombinant Human DFFA Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032359-10, PKSH032359-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: DFFA
Target Synonym: DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor 45 kDa Subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13
UNIProt ID: O00273
Research Areas: Cell biology; Cancer; epigenetics and nuclear signal; metabolism
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: O00273
Background: DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.
Sequence: Met 1-Thr331
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 38.7 kDa
Observed MW: 42 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only