Recombinant Human HER2/ErbB2 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031997-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: HER2; ErbB2
Target Symptom: Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1; CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; TKR1
Research Area: Signal Transduction; Tags & Cell Markers; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_004439.2
Background: Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Activity: Immobilized human Erbb2 at 2. 5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1. 28-32 ng/ml.
Sequence: Met 1-Thr 652
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 71 kDa
Observed MW: 100-110 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only