Recombinant Human IL-10/Interleukin-10 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032005-10, PKSH032005-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-10; Interleukin-10
Target Synonym: Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10; RP11-262N9.1; IL10A; MGC126450; MGC126451; TGIF
UNIProt ID: P22301
Research Areas: Cancer; immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: None
Accession: P22301
Background: Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted protein and
belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic
stellate cells, keratinocytes, and placental cytotrophoblasts. IL-10 is an anti-inflammatory TH2 cytokine
that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in
produced in several T helper populations, it is proposed that it provides a feedback loop to limit the
effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10
receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable
biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In
addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral
infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible
clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.
Sequence: Ser19-Asn178
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19.6 kDa
Observed MW: 16 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only