Recombinant Human PCBD1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032981-10, PKSH032981-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PCBD1
Target Synonym: Pterin-4-Alpha-Carbinolamine Dehydratase; PHS; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Dimerization Cofactor of Hepatocyte Nuclear Factor 1-Alpha; DCoH; Dimerization Cofactor of HNF1; Phenylalanine Hydroxylase-Stimulating Protein; Pterin Carbinolamine Dehydratase; PCD; PCBD1; DCOH; PCBD
UNIProt ID: P61457
Research Areas: Signal Transduction; epigenetics and nuclear signal; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P61457
Background: Pterin-4-α-Carbinolamine Dehydratase (PCBD1) is the founding member of the Pterin-4-α-Carbinolamine Dehydratase Family. PCBD1 is involved in Tetrahydrobiopterin biosynthesis. It seems to prevent the formation of 7-Pterins and accelerate the formation of Quinonoid-BH2. Furthermore, PCBD1 regulates the homodimerization of the transcription factor Hepatocyte Nuclear Factor 1 (HNF1) and enhances its transcriptional activity. Defects in PCBD1 are the cause of BH4-Deficient Hyperphenylalaninemia Type D (HPABH4D). HPABH4D is characterized by the excretion of 7-substituted Pterins in the urine of affected patients.
Sequence: Ala2-Thr104
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14.2 kDa
Observed MW: 14 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only