Product Description

RhoA Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67042-50, MB67042-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Rho family small GTPases, including Rho, Rac and cdc42, act as molecμlar switches, regμlating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regμlation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) . RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellμlar localization allow these three proteins to respond to and act on distinct signaling molecμles .Functions of RhoA, the most highly studied of these three, include regμlation of actomyosin contractility , cytokinesis , focal adhesion assembly and cell polarity .

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB

Alternate Names: ARH12, ARHA, RHO12, Transforming protein RhoA, Rho cDNA clone 12, h12

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human RhoA. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/8000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of RhoA protein.

Molecular Weight: ~ 22 kDa

Pathway: Regμlation of Actin Dynamics, Adherens Junction signaling, Regμlation of Microtubμle Dynamics, SAPK JNK Signaling Cascades, TGFβ Smad signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P61586

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only