Product Description

Rictor (Phospho-Thr1135)  Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65995-50, BS65995-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cell growth is a fundamental biological process whereby cells accumμlate mass and increase in size. The mammalian TOR (mTOR) pathway regμlates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for fμll Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regμlates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF, IHC

Alternate Names: Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR, KIAA1999

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Rictor around the phosphorylation site of Threonine 1135.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rictor (Phospho-Thr1135) polyclonal antibody detects endogenous levels of Rictor protein only when phosphorylated at Thr1135.

Molecular Weight: ~ 210 kDa

Pathway: PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q6R327

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only