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RmesFect™ Stem Transfection Reagent
RmesFect™ Stem Transfection Reagent

RmesFect™ Stem Transfection Reagent

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$205.00
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RmesFect™ Stem Transfection Reagent is based on the TEE-technology specifically designed for mRNA transfection in Stem Cells with high efficiency and low toxicity. mRNA transfection provides several advantages over plasmid DNA delivery :

  • No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm
  • Faster protein expression than DNA transfection
  • No genomic integration
  • Protein expression in a total promoter independent manner
RmesFect™Stem is specifically designed for in vitro mRNA (dsRNA) transfection in a large variety stem cells and primary cells.

 

RmesFect™Stem transfection reagent principal advantages:

  • Highly efficient : achieve mRNA delivery in all cells
  • Ready-to-use: no need for additional buffer
  • Low nucleic acid amount : minimized toxicity
  • Protects mRNA against degradation
  • Compatible with any culture medium
  • Serum compatible : Medium changed not required

Sizes:

  • 500µL (RS30500): 125-250 transfections with 1 µg of mRNA
  • 1 mL (RS31000): 250-500 transfections with 1 µg of mRNA
  • 5 mL (RS35000): 1250-2500 transfections with 1 µg of mRNA


Storage: - 20°C
Shipping Conditions: Room temperature

 

Application

  • Ideal for your mRNA transfection applications in stem cells:
    • mRNA vaccines
    • Embryonic and multipotent stem cells transfection
    • Regenerative medicine
    • Faster cell reprogramming : allowing daily transfections for iPS generation

RECOMMENDED FOR: Transfection of mRNA in stem cells.

 

Results

Rmesfect Stem Transfection results

Figure 1: Several Stem cells were transfected using RmesFect Stem. Complexes were prepared as followed: mRNA encoding GFP protein (0.25 μg for human hMSC and 0.5 μg for MEF and hAdSC) was mixed with RmesFect Stem transfection reagent (ratio 2:1 for hMSC and 4:1 for MEF and hAdSC). After 20 min of incubation at room temperature, the complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by  FACS analysis.

 
RmesFect Stem Transfection efficiency

Figure 2: Several Stem cells were transfected using RmesFect Stem. 24 H after, transfection efficiency was measured by fluorescence microscopy.