RNF128 Polyclonal Antibody-BS65454

RNF128 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65454-50, BS65454-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q8TEB7

Host: Rabbit

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Zebrafish,

Applications: WB, IHC-P

All Applications: WB=1:500-2000 IHC-P=1:100-500

Background: The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RNF128 Polyclonal Antibody detects endogenous levels of RNF128 protein.

Bioworld Molecular Weight: 43kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti- RNF128 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Alternative Name: E3 ubiquitin protein ligase RNF128; E3 ubiquitin-protein ligase RNF128; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128; RN128_HUMAN; RNF128.

Immunogen: KLH conjugated synthetic peptide derived from human RNF128:151-260/428

Conjugate: Unconjugated

Modification: Unmodified