Product Description

ROCK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16583-50, BZ16583-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: Q13464

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF, IP, , FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: ROCK1 Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 158 kDa; Observed MW: 158 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ROCK1 in Ramos lysates using ROCK1 antibody., Immunohistochemistry analysis of paraffin-embedded Human kidney using ROCK1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of ROCK1 in Hela using ROCK1 antibody.

Alternative Name: ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK

Immunogen: A synthesized peptide derived from human ROCK1

Conjugate: Unconjugated

Modification: Unmodified