Product Description

ROM-K (phospho Ser44) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6002-50, ES6002-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF, ELISA

Alternate Names: KCNJ1, ROMK1, ATP-sensitive inward rectifier potassium channel 1, ATP-regulated potassium channel ROM-K, Inward rectifier K(+) channel Kir1.1, Potassium channel, inwardly rectifying subfamily J member 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ROMK/Kir1.1 around the phosphorylation site of Ser44/25. AA range:11-60

Isotype: IgG

Target: ROM-K

Specificity: Phospho-ROM-K (S44) Polyclonal Antibody detects endogenous levels of ROM-K protein only when phosphorylated at S44.

Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: In the kidney and pancreatic islets, Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver,

Molecular Weight (Da): 45kD

Fields: Aldosterone-regulated sodium reabsorption, Gastric acid secretion

Function: Disease: Defects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) [MIM: 241200], also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia
Function: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by

Subcellular Location: Cell membrane, Multi-pass membrane protein Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane

Storage and Stability: -20°C/1 year

Gene Name: KCNJ1

Protein Name: ATP-sensitive inward rectifier potassium channel 1

Mouse Gene ID: 56379

Mouse SwissProt Number: O88335

Rat Gene ID: 24521

Rat SwissProt Number: P35560

Research Use Only