Product Description

RPS6 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67048-50, MB67048-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregμlating mRNA translation . Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions . These particμlar mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation . Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein .

Category: Primary Antibody

Reactivity: Human, Rat

Host: Mouse

Applications: WB, IHC, IF/ICC, FC

Alternate Names: 40S ribosomal protein S6, Phosphoprotein NP33

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human RPS6. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of RPS6 protein.

Molecular Weight: ~ 32 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P62753

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only