Product Description

RSK3 Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66411-50, MB66411-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains and a carboxy-terminal docking site for extracellμlar signal-regμlated kinases (ERKs). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation . RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters. Upon mitogenic stimμlation, p44/42 ERK1/2 and ERK5 MAP kinases cooperatively phosphorylate p90RSK Thr573 (p90RSK1 numbering) located within the C-terminal kinase domain and Thr359/Ser363 in the linker region between the two kinase domains. Phosphorylation of Thr573 within the activation loop of the p90RSK C-terminal kinase domain promotes activation and directs phosphorylation of Ser380 within the hydrophobic stretch of the linker region. The phosphorylated p90RSK Ser380 acts as a docking site for the constitutively active Ser/Thr kinase PDK1, which in turn phosphorylates Ser221 within the N-terminal kinase domain activation loop, resμlting in fμll enzymatic activation of the p90RSK. Antibodies against these phosphorylation sites are usefμl for understanding the kinetics and regμlation of p90RSK activation.For more information regarding the phospho-regμlatory sites within each RSK isoform, including more information regarding the seminal studies demonstrating the complex phosphorylation cascades involved, please see the references herein and PhosphoSitePlus.

Category: Primary Antibody

Reactivity: Human, Hamster

Host: Rabbit

Applications: WB, IHC, IF/ICC, IP

Alternate Names: MAPKAPK1C, RSK3, Ribosomal protein S6 kinase alpha-2, S6K-alpha-2, 90 kDa ribosomal protein S6 kinase 2, p90-RSK 2, p90RSK2, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, MAPKAP kinase 1c, MAPKAPK-1c, Ribosomal S6 kinase 3, RSK-3, pp90RSK3

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human RSK3

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of RSK3 protein.

Molecular Weight: ~ 83 kDa

Pathway: Jak Stat: IL-6 Receptor Signaling, Cell Cycle G2 M DNA Damage Signaling Pathway, Regμlation of Apoptosis, MAPK Erk in Growth and Differentiation Pathway, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, MTOR signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15349

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only